POSITION RESPONSIBILITIES:
Assist Dr.
Robert Eddy, Administrative Director of the Clinical Prognostics Program (CPP), with training and management of IIPCR program personnel including research technicians, graduate students, post-doctoral fellows, T-32 surgical fellows.
Manage the preparation and execution of an in vitro invadopodia assay to screen, in breast, PDAC and lung cell lines, drug inhibitor candidates of metastasis, generated by the Therapeutics Development Program (TDP) targeting Mena and Pyk2.
Maintain mouse colonies that will be used to test drug inhibitor candidates of Mena and Pyk2, and Tie2 and CSF1R inhibitors (from Pharma) for effects on overall survival of transgenic and orthotopic mouse models of breast, PDAC and Lung cancer.
Oversee a custom-designed, affinity purification of a MenaINV polyclonal antibody used by the CPP Translational Pathology Laboratory and IIPCR clinical programs to predict tumor cell invasiveness.
Work with the Therapeutics Development Program (TDP) under the direction of Dr.
J.
P.
Maianti in the development and implementation of Mena and Pyk2 inhibitor target-engagement screening assays including:
PROTAC-induced inhibition and Western blotting
HiBiT-tag reporter cell-line and LgBit nanoluciferase assay
NanoBRET protein-protein interaction assay.
Work with the IIPCR clinical coordinator, Dr.
Maria Brown, in preparation of patient blood samples for flow cytometry analysis (buffy coat prep).
In collaboration with CPP, work on further optimizing TMEM doorway staining to increase its compatibility with automated analysis.
This will enhance the likelihood of transferring TMEM doorway score to clinical applications.
Assist IIPCR Breast Program post-doctoral fellow, Anastasia Chondronikola, in generating ER positive breast cancer cell lines expressing stem SORE6-GFP reporter and testing the induction of stemness under various conditions in vitro.
Help IIPCR Breast Program personnel including graduate students, post-doctoral fellows, and T-32 surgical fellows in performing and reading transwell assays (intravasation-directed transendothelial migration [iTEM] and extravasation-directed transendothelial migration [eTEM].
Help IIPCR Breast program personnel including graduate students, post-doctoral fellows, and T-32 surgical fellows in troubleshooting any of the assays that you are familiar with, and they are not.
Also, you will work with Dr.
McAuliffe, leader of the Pancreatic Cancer Program in the IIPCR regarding mouse models of pancreatic ductal carcinoma progression and Dr.
Oktay, leader of the Breast Program, regarding items 3-7 above.
You will report directly to Drs.
Eddy, McAuliffe and Oktay.
QUALIFICATIONS:
Bachelor's degree with 5+ years of experience
Salary Verbiage: This outlines the salary range for exempt and non-exempt positions Minimum Salary Range: USD $85,000.
00/Yr.
Maximum Salary Range: USD $93,738.
00/Yr.
Whatsapp
